7. The effluent from a treatment plant may not show acute toxicity for rainbow trout Oncorhynchus mykiss or daphnia Daphnia magna, or both. Acute toxicity corresponds to a mortality rate of more than 50% of the organisms exposed to the undiluted effluent.
The operator of a medium, large or very large treatment plant must perform the acute toxicity tests provided for in Schedule II in accordance with the frequencies and procedure listed in that Schedule.